Iago’s soliloquy at the end of Act 1 Essay

â€Å"Iago’s soliloquy at the end of Act 1; what does his language tell us about his character and motivation? How does it compare with his language in the rest of the act†? Iago seems to be presented as a Machiavellian villain; he is cunning and always seems to know what’s going to happen. In Iago’s soliloquy at the end of Act 1 Scene3, he says of Roderigo â€Å"thus do I ever make my fool my purse†. This conveys Iago’s character as superior and manipulative. Iago states that Roderigo is a â€Å"fool†; a stupid moron. He also calls him a â€Å"snipe† which is a small bird which also is used to mean unintellegent. Iago refers to Roderigo possessively, referring to him as â€Å"my fool† as if the extent of his own influence makes Roderigo his own possession (as with â€Å"my purse†; purse being an object that is owned). By saying â€Å"I even make† Iago is implying that manipulating a â€Å"fool† for their money is a usual activity for him, as if he always does this. Iago holds such little respect for Roderigo and feels himself so superior that he â€Å"should profane if [he] time expend with such †¦ But for [his] sport and profit†. He’s claiming that Roderigo is so beneath him that it is only for the money (â€Å"profit†) and the game he plays with the characters (â€Å"sport†) that he’d ever bother wasting his time with such an idiot. This seems to be revealing of Iago’s attitude toward social classes. Just because another character is richer or has higher social standing this does not mean that he has any extra respect for them. Taking into account that England in the Elizabethan era worked with strict social classes I think that Shakespeare uses Iago’s lack of respect for the system as another way of demonising him. He is the villain because he believes himself to be superior to everyone else. Iago is Othello’s ‘ancient’. However, Iago obviously feels he is superior to his master. Iago likens Othello to a donkey; a dull, stupid animal. Iago says Othello will â€Å"be led by th’ nose. As asses are†. Asses, or donkeys, are literally led by the nose with a harness. Might the harness be the society they are both part of? This implies that Othello is not free. It implies that he is tamed, obedient, dependent and without a mind of his own. It is Iago’s intention to use this harness to lead Othello to his ruin. In act 1 scene 1 Iago reveals his views on the roles of master and servant (in his case ancient) to Roderigo. Iago’s opinions show his perceived superiority in his character. Iago says how there are â€Å"many a duteous and knee crooking knave that†¦wears out his time, much like his master’s ass†. He is saying that the dutiful are â€Å"knee crooking†, meaning that they bow down, accepting their inferiority. To say that a subordinate â€Å"wears out his time much like his master’s ass† shows how he feels that they waste their lives being another’s workhorse while receiving none of the profits. In this respect Iago feels himself above Othello. By later referring to Othello as an â€Å"ass† he could be the â€Å"knee crooking knave† to the governors of Venice. This is what I feel is supposed to be conveyed by the line: â€Å"were I the Moor, I would not be Iago†. Iago is resentful of the lack of recognition he has received from his society. I would say that Iago has motivation against his society. He misses out on promotion and Cassio takes the position. He resents Cassio for being better educated and of higher social standing. From scene 1 Iago says â€Å"I know my price, I am worth no worse a place† when telling Roderigo of being passed over for promotion. I think that Shakespeare has Iago say this because he’s supposed to be resentful of the lack of recognition he’s received. By saying â€Å"I know my price† he is also saying that no-one else perceives his worth. Iago mentions that Cassio is â€Å"a Florentine† while disrespectfully describing him. That Shakespeare has Iago mention this means that it is relevant. Perhaps that Iago disapproves of a Florentine being promoted in a Venetian army shows he has a kind of respect for the society he’s in. If he is ambitious then he is ambitious toward the higher roles/accomplishments of his own society; Venice. Iago may also feel he has not been duly acknowledged for the fighting he has done for the causes of Venice â€Å"at Rhodes, at Cyprus, and on other grounds Christian and Heathen†. By not being advanced he may have felt the sacrifices he made were not appreciated, as if he’d been cheated, which may explain why he cheats so much in the conventions of his society. Iago is presented as being a very effective user of language. He seems to know exactly the right language to use in order to affect the decisions of the other characters. When bating Brabantio he uses course language about his family to infuriate him. Instead of merely informing Brabantio of his daughter’s whereabouts and who she is with Iago tells him that â€Å"your daughter and the moor are now making the beast with two backs†. â€Å"Making the beast with two backs† is a crude euphemism for having sex. â€Å"Beast† implies that the sex is ugly and savage. Iago uses the word ‘moor’ instead of his name, Othello, to bring attention to his race as opposed to his high rank and standing in Venice. Iago knows how to offend. He immediately starts referring to Brabantio’s family in animal terms; â€Å"you’ll have your nephews neigh to you; you’ll have coursers for cousins and jennets for germans†. In the Elizabethan era it was probably a taboo to have a mixed race marriage probably because people of African origins would have been considered inferior. This is a reason why Iago refers to Othello as a horse (â€Å"coursers for cousins†). As a further example of Iago’s ability to alarm through his seemingly perverted perception would be â€Å"you’ll have your daughter covered with a Barbary horse†. Iago alarms him by conjuring bestial imagery. Bestiality is sacrilegious, which a few hundred years ago was more important than it is now. Perhaps this sacrilegious imagery influenced Brabantio to rationalise his daughter’s behaviour as witchcraft. Shakespeare presents Iago as an effective liar. This must be the case as Othello refers to him in Act one as â€Å"Honest Iago†. He also describes him as â€Å"a man he is of honesty and trust†. Despite Iago keeping Roderigo’s presents to Desdemona for himself he still can convince him of his trustworthiness. When reassuring Roderigo he says â€Å"I have professed me thy friend, and I confess me knit to thy deserving with cables of perdurable toughness†. By claiming that he is Roderigo’s ‘professed’ friend and that he’s prepared to help him with everlasting strength he convinces of his honesty. Iago also successfully manipulates Roderigo by repeatedly suggesting (instructing really) to â€Å"put money in thy purse† so as he can take it from him. Iago repeats this six times. Iago also convinces Roderigo to do his biddings by distracting him with his philosophies; â€Å"Our bodies are our gardens, to which are wills are gardeners†. In this speech Iago basically turns Roderigo’s loss into his own gain.

Outcomes of the Crusades

The â€Å"Crusades† was a military campaign of Christians in Western Europe whose purpose is to reclaim the Holy Land from the Muslims. The Roman Catholic Church stimulated most of the support for the war, showing its intolerance to both Islam and Orthodox Christianity. Although religious in nature, the Crusades had become a bloody frenzy. Six military campaigns comprised the period, starting from the First Crusade in 1096, inspired by Pope Urban II, and ending in the Sixth Crusade in 1254, led by the king of France, King Louis IX.To this day, the Crusades is still a controversial because of the military excesses during the battles. However, many outcomes came out of the movement: First, the European horizon expanded; second, the encounters with the different cultures promoted learning as well as commerce between different civilizations; third, as a result of the eastern influence, there was growth in western scholasticism and philosophy; and fourth, it sparked a dangerous con cept adapted in monarchy and politics. Among the noted significance of the first crusades was the increased contacts between the Europeans and the Byzantines and Muslims.During this time, the Byzantine was already beginning to decline and Islam was not as powerful as it once was, although still a formidable force. As regards the original intention of the Crusades, the Christians' success of having Jerusalem under their control never reached permanence. However, the first crusades effected the expansion of Europe, meaning it gained more strength than other competing civilizations did. Moreover, there became a balance of power between the Muslims and the Christians.During the First Crusade, supporters of the movement, such as those from Pisa and Genoa, sailed the Mediterranean Sea to bring help to Jerusalem. As a result, the sea was again reopened to western shipping, and, in turn, communication was reestablished between the east and west. Although at several points the Christians rul ed over the Holy Land, Muslims were able to conquer it again in the 12th century. However, the Christians continued to hold power over the sea. Thus, the ports in the countries such as Levant in the border of the sea were under their control.The number of commercial establishments grew rapidly in the ports of Syria and Egypt, and the Christians held autonomy over operations in the areas of Corsica, Sardinia, and Sicily, which were originally controlled by the Saracens (Riley-Smith 85). The sea routes were open for trade, allowing products of Asia to come in. In short, the Crusades opened the lines of communication and exchange of knowledge between the eastern and western civilizations through the trade. This made a significant contribution to the development of Europe in terms of both knowledge and economy.Some of the practices of Muslim science, literature and philosophy, as well as medicine had found its way to Christian tradition (Riley-Smith 87). In time, the crusading movements were divided into two groups: the external Crusades and the internal Crusades. The former was directed mainly against Muslims, whereas the latter was a war against the perceived enemies of the Christian world. Unfortunately, the development of the internal Crusades enforced a violent thinking—that is, violence is sanctified in ideological pursuits.The Crusades was originally a war to retrieve the Holy Land, but the concept of sanctified violence extended to the monarchy and the political sphere. What used to be the holy cause of defending the Church became a moral duty of defending the state (Riley-Smith 90). Indeed, the Crusades was a crucial moment in history, and one that people can learn from. That is, the good things that came out of it should be emulated, and its detrimental effects should serve as a warning to modern society. Works Cited Riley-Smith, Jonathan. â€Å"The First Crusade and the Idea of Crusading†. Philadelphia: University of Pennsylvania, 1991. 85 -96.

Analysis Of The Book The Circle

Dave Eggers’ The Circle, it set certain reverberations echoing in my mind, especially through the correlation of The Circle’s three slogans, â€Å"SECRETS ARE LIES, SHARING IS CARING, PRIVACY IS THEFT† (Eggers 305), with 1984’s â€Å"WAR IS PEACE, FREEDOM IS SLAVERY, IGNORANCE IS STRENGTH† (Orwell 6). I then began to see, like others, that Eggers’ dystopia is a contemporary 1984; therefore, I felt it would be appropriate to compare them. In almost all the reviews and essays written on The Circle, there is not†¦ Grand Central Publishing released Flora Rheta Schreiber’s book Sybil. Through the book Dr. Wilbur, Sybil’s therapist psychoanalyses Sybil’s sixteen different personalities. The book went through all the different personalities and what is the cause of her dissociation. After her analysis was complete Dr. Wilbur thought a book should be written, this was when Flora was introduced into the picture. Flora had had previous work as a journalist and editor of varying psychiatric papers and articles. After†¦ The Hughes Brothers imprint their religious point of view in their 2010 American post-apocalyptic film, â€Å"The Book of Eli†. In this dramatic tale Denzel Washington stars as Eli, a mysterious wanderer that carries the world’s only remaining copy of King James’s Bible. He ventures 30 years across a desolated post-apocalyptic landscape in the attempt of finding a safe haven for this sacred book. Determined to complete his mission, Eli’s quote â€Å"We walk by faith, not by sight† from the Bible conveys a†¦ J07 -- THE BOOK OF TELLS 1 Citation. Collett, P. (2003). The book of tells: How to read people’s minds from their actions. London, United Kingdom: Doubleday. Tells are a recognizable action or a method of performing an action that reveal something about a person that is not otherwise easily observable (Collett, 2003, p.15). The Book of Tells, written by social psychologist Dr. Peter Collett, decodes tells to explain what these subtle clues of human behaviour and motivations are telling us. By delving†¦ establishing and emphasizing whatever specific emotion that the artist wishes to portray. It can also signify a call for extra attention from the reader to a specific page, as the line work suddenly changes in style between pages. In â€Å"The Outside Circle†, the line work on the 26th page is conveying the shattered life and transitioning that the character Joey Caver is going through, and is a visual representation of his emotions negatively being broken down. Lines are everything within a comic as†¦ Major Works Review General 1. Title- The Book Thief 2. Author- Markus Zusak 3. Date of Original Publication- 2005 4. Novel Type- Historical Fiction Structure 1. Point of View- Death 2. Relationship to Meaning- As an omniscient force Death can provide insight on several different events happening all over the world at different points in time, and thoughts running through all the characters’ heads, while also being able to share his own thoughts and feelings about humanity. 3. Plot Structure- a. Exposition-†¦ transporting them. As a result between with a death of 9.6 and 10.8 million Africans arrived in the Americas alive. With the odds against Aminata Diallo, she faces many losses but through these losses Aminata manages to re-defines herself. In "The Book of Negroes" the novel focuses on Aminata 's self-discovery through her many loses, including her loss of innocence, the loss of safety, and the loss of relationships. Aminata first losses her innocence at the age of 11 when colonist invaded her small†¦ Comparatively: A Review Circles are globally ubiquitous. They occur naturally, are used for style, and used as metaphors. One metaphorical use of circles is found in Zen Buddhism. An EnsÃ…  is a hand drawn circle, generally in one brush stroke. It is elegant in its simplicity and symbolises enlightenment, the void, and the wholeness of the universe. In Peter Singer’s book The Expanding Circle, he argues that moral progress comes when people use their reason to progressively expand their circle of empathy from†¦ The novel, The Book Thief, was published in the year 2005 and falls under the genre of historical fiction. In the novel, death narrates the story beginning in 1938. Liesel Meminger is presented as the book thief and is faced with many hardships at the beginning of the novel. She has lost both her parents including her little brother, Werner. This girl is faced with agony and death at such a young age in her life. This forms Liesel into a person who isolates her inner humanity and compassion towards†¦ According to Psychology Today, on average kids spend 5-7 hours a day looking at screens. This is a staggering amount, accounting for around half of daylight hours. In The Circle, a novel by Dave Eggers, a young girl named Mae in a job with a large technology company spends close to her entire day on a screen, leaving little time for interactions with people. Instead she relies on how others perceive her online, rather than as a real human being. Truman’s entire life is fake in The Truman Show, a†¦

Observe different person's behaviors in hospital Essay

Observe different person's behaviors in hospital - Essay Example From a theoretical point of view, the behavior shown at work is the result of operant conditioning reinforced through punishment and rewards. Therefore, individuals’ behavior can be assessed well only if they are approached from various dimensions. It is interesting to note that people who gather at a public place are more likely to express their real traits when compared to those at workplace. I spent some time observing how the patients and staff at Miami Children’s Hospital behave in the hospital premises. At the conference hall, I observe the parents or relatives of some patients attending Professor Juan Aqua’s orientation speech on ‘Childrens Medical Services Primary Care Program’ (CMS). He gives lectures on the significance of the program emphasizing on how the Miami Childrens Hospital Pediatric Care Center serves as a medical home for their child once enrolled in the Program. His old age and his low voice make it extremely difficult for audience to catch what he says. Evidently, he had this speech earlier perhaps for another batch, for he speaks to the audience as if they are already aware of the program. The staffs at the venue show no special interest in the speech as they are familiar with each and every aspect of the CMC. People attending the talk have multiple responses. Some are interested to hear what the professor says whereas those sitting in the last rows look distracted. Obviously, they are happy to get the professor to lecture from the middle of the class in a much louder voice. They may pay better attention to the professor’s lectures, and answer his questions only if the professor delivers lectures in a louder voice in an interactive way. Many of them remain unresponsive, waiting for their turn to move to the registration counter. The orientation program is mandatory for enrolment. However, there are a few parents who take notes of each

Is the death penalty effective (argument) Essay

Is the death penalty effective (argument) - Essay Example Different studies conducted in different states have concluded differently about the deterrent effects of death penalty, which makes it difficult to deduce the effectiveness of capital punishment (Durlauf, Fu, and Navarro 1). Despite the subjectivity of its effectiveness from statistical observation, and the fact that death penalty is the harshest of all punishments that can be possibly given to the criminals, its usefulness and effectiveness in establishing peace in the society cannot be denied. Spending millions of dollars on death penalty is worth it because it is really effective in reducing crime rate, establishing the right justice system, and inculcating fear of law in the criminals. Capital punishment eradicates the possibility for the same criminal to reengage in crime ever. It is as simple as this; no more criminal means no more crime. Every criminal that is released from prison after completing the assigned duration of imprisonment carries the possibility of reengaging in crime. In fact, many criminals do reengage in crime. After their release, they are exposed to the same environmental, social, and cultural factors that convinced them to commit the crime before. This is the reason why many criminals are returning prisoners. Death penalty provides the most effective solution against crime recurrence. There is no recompense as fair as death penalty for certain crimes. The relatives of a person who has been murdered mostly want nothing less than murder of the murderer. The criminal deserves to be given death penalty because he/she has caused the same to someone else; â€Å"if those murdered are the sort inclined to forgive those who injure them, their murderers can take comfort that on dying they might be forgiven and at one, in the sense of having a morally balanced relationship restored, with their victims† (Aspenson 104). Fair judicial system demands that a criminal is granted punishment commensurate with the weight of the

Personal statement for admission to law school Essay

Personal statement for admission to law school - Essay Example By the time I reached my junior year in high school, I had become more appreciative of learning and studying the World History particularly sparked my interest to engage in debates and forums in and out of school. I started being passionate in expressing my views on several issues of concern which prevailed at the time and my growing fascination for language and communication had all the more encouraged me to attain further awareness in social and political aspects of living. I must admit though that I encountered occasions when interactions were weaving unhealthy arguments instead. In the process, however, I learned to find worth in listening to others with downright humility and how essential it is to be working on relationships that bear positive impact despite inevitable competitions with batchmates. This principle has supported me to yield into a more flexible character that seeks to understand with patience and sincerity even to this day. During early college and the three year s of attendance in the Danube International School, I spontaneously developed love toward philosophical studies which deepened my academic inclinations and involvement with other avenues of learning where public speaking matters with ample essence. Some fellow students under a similar or related field also influenced me to find delight in studying classical literature and poetry which serves as a collective inspiration for my creativity in thought and manner of speech. I recollect having looked up to certain intellectual figures covered in the literary courses and through them, I felt the necessity to contemplate on the real worth of wisdom and acquisition of a revolutionary mind from which acts of profitable reform may be derived to be taken advantage of by the society as a whole. Stepping out of the academe via extracurricular commitment gave me the opportunity to explore communities under crises of varied types. There used to be times when I participated in reach-out projects whi ch were sponsored either by our school or the government and such enlightening experience truly makes a huge difference in one’s perception of life. When we paid visit to poor families, my heart could not help outpouring cries at the view of poverty and a general picture of hopelessness. I needed no effort then to empathize for it was such a revealing moment of truth when reality unfolded itself to break my core and let loose all of the inherent compassion within. At this stage, I told myself that I knew exactly and could discern what my professional desire was going to be like. Due to the actual sights and physical encounter of impoverished communities, the idea of injustice came instantly and I was brought to ponder that such state of economy has chiefly suffered from inequality especially in terms of allocation of goods. For a case as this, there is much that the government could in the absence of corruption or illegal activities. Hence, I decided to pursue law in order th at by securing a position as a lawmaker, I would be able to earn the authority of defending people whose rights

Jehovah's witnesses Case Study Example | Topics and Well Written Essays - 750 words

Jehovah's witnesses - Case Study Example This discussion seeks to apply the provisions of the Case Treatment of Jehovahs witnesses, to analyze the issue of abortion. While the Jehovah’s witnesses have a strong stand against receiving any form of treatment for any member of their community, no matter whether such members are adults, teens or children, the issue of abortion is completely different. While approached from the religious perspective, children are a gift from God, whose life does not start when they are born but rather at the time they are conceived (Beckwith, 47). In this respect, the procurement of abortion is the same as committing murder, since it amounts to killing a living human being. In this respect, the practice of abortion is an evil that the society should not commit. Thus, the society should also treat the issue of abortion the same way the Jehovahs witnesses treats the issue of medical treatment. According to the Case Treatment of Jehovahs witnesses, Medical treatment for the Jehovahs witnesses is an issue that is highly prohibited and authoritative, and which may lead to many unintended consequences. For the Jehova hs witnesses, treatment is not allowed may it be for adults, teenagers or children, making it highly unlikely that abortion in whichever format would be acceptable in this society. In the case, Georgette Malette was a 57-year old woman who sued a physician for the treatment that saved her life. She received a blood transfusion which was necessary to save her life after she was engaged in a road accident that had killed her husband. Since she was unconscious, the physician administered blood transfusion oblivious of the fact that Georgette had a card on her purse that required that no blood should be administered under any circumstances. Thus, despite the fact that Georgette’s life was only saved by the blood transfusion she received from

Nationalism in Italian and German Unification, 1815-1871 Essay

Nationalism in Italian and German Unification, 1815-1871 - Essay Example Particularly in the smaller states the prince remains the embodiment of national ideals. This dynastic loyalty implied by the seventeenth century, in any case, the works of a prevalent national consciousness concentrated though it could be upon the governing divine monarch. All over Europe the mercantilist scheme put emphasis on the precedence of national consciousness and interest over those of other nations. Nationalism assumed on various forms as it infiltrated different ways of observing the world and the nation. There is no solitary nationalism; there is in its place a diversity of nationalisms. Furthermore, these nationalisms developed within the paradigm of the varied European countries. However, in this development we can make out a number of common milestones. Otto Van Bismarck of Germany, the genuine politician, approached the front, whereas in Italy the ambitious Giuseppe Mazzini was succeeded by the pragmatic Camillo Cavour, whose preferred philosopher was Jeremy Bentham, a renowned Utilitarian (llobera 1994). National aspirations were guided toward practical accomplishments through peacekeeping or military hostility. This was merely one transition in nationalism after 1848, nonetheless; there was another. This essay will attempt to discuss a comparative point of view on the form of nationalism that developed in Italy and Germany in the Unification attempts initiated in the nineteenth century.

Real Estate Market Crisis Essay Example | Topics and Well Written Essays - 2000 words

Real Estate Market Crisis - Essay Example It is because of this reason that various banks and financial institutions have developed their internal rating methodologies which they assign to various consumers asking for credit. Banks than through their internal as well external credit scores or ratings decide to whom they should provide the credit. These criteria of credit rating often are designed to be tough since Banks in order to avoid defaults, tighten their criteria to extend the credit to these customers. However, due to increasing needs of those customers, whose credit history or their credit ratings do not fall under the criteria laid down by the banks, banks try to accommodate them also. Sub-prime lending is the part of that phenomenon. A subprime lender provides loans at higher interest with inappropriate incentives to high risk borrower. Ideally a subprime lender is one who lends to the borrowers who do not qualify for the loans from mainstream lenders. This is because of the fact that those borrowers who have a bad credit history. A subprime lender is mainly an independent entity or the sub-entity of the main players in the markets. However the only way to identify the lenders who lend to the subprime borrowers is to judge from their prices as they are normally higher than the prices charged by the normal lenders with standardized terms and conditions in the market. A subprime lender generally lend following types of loans: i) Interest Only Loans are the loans which give borrower an option to pay interest on their loans only; thereby Principal remain unchanged and at the end of loan term payments increase substantially. Traditionally these loans are cheaper in nature as the normal mortgage loans require some portion of principal and mark-up to be paid in the monthly installment of the mortgage. These loans became popular mainly due to the fact that they required lower loan payments therefore buyers can afford the larger homes as

Creativity report Coursework Example | Topics and Well Written Essays - 1250 words

Creativity report - Coursework Example Creativity and innovation have become valuable features of leading companies. Schirrmacher (1988) defines creativity as one’s ability to see things in novel ways; it is breaking boundaries and going beyond the known facts. It is also developing something new, different and original and combining things which do not seem to be related to each other but eventually makes sense when put together. This is closely related to innovation which is sparked by a new idea. The idea can be a new technology such as a gadget that can provide more convenience to consumers, a new service like an alternative form of relaxation, a new product, or even a new administrative procedure. Such ideas are driven by needs that are seen by the innovators. Innovation requires creativity. In organizations, innovation becomes the growth of a new idea from its initial state into its actualized form as a full-blown innovation (Roberts, 2006). Thus, creativity is at work when one innovates. The ongoing global recession has caused the shutdown of several companies, leading to massive unemployment. Epic Events Management Company is a small company that has likewise suffered the blow, with many of their clients opting to forgo their special events as a way of cutting down expenses. To keep the company afloat, they had to let go of a large part of their office space which occupied the whole floor of a prominent building downtown. The CEO decided to just keep his office for entertaining clients, the team room with a few office tables and the reception area. Because of this drastic move, they have significantly saved on rental expenses. The problem now lies on how they would rearrange the office so that all the staff can fit in and still be productive. On top of that, they need to think up of other alternative income-generating ideas that will augment the losses incurred from the lack of projects. For the problem of lack of office space for the

Authorship of the book of Ruth Essay Example for Free

Authorship of the book of Ruth Essay The book of Ruth has been historically claimed to be written by the prophet Samuel. It dates back to the 1011 and 931 B. C. It was a message of the love of God directly to the Israelites. With the main theme of sacrifice, it was essential for them at that time to hear such words of love. In the heathen land of Moab, located North of the Dead Sea, Ruth is resides for a period of time and them moves further into the town of Bethlehem, during the era of the judges which occurred directly after the Pentateuch books which are the first five biblical books. Ruth was the daughter-in-law of Naomi and Elimelech, who were an Israelite couple with two sons. Mahlon and Chilion were their names and both married Ruth and Orpah. Sadly, all three men in the family died, firstly Elimelech and then later on his two sons, leaving three widowed women. The actual period is not actually stated. However, most scholars have attributed its authorship to the pre-exilic era; the period after the enslaved Israelites were freed and permitted to return back to their homeland of Israel. As a historical book of the Bible and an excellent piece of literature, it has challenged the conception of the era and the norms of the periods as recording the attitudes and conducts between people. It demonstrated tremendous practicality and dealt with serious relational issues and we can learn a lot from it today. Its authorship is unknown. It clearly begins by alluding to the period of the judges and it is perceived by some to have been written around that time. The prophet Samuel has been given the credit for its authorship by many scholars. In recent times however, there have been speculations as to the real author of this book. It has been recognised as having been written by a female author. According to an ancient Beraithato the Talmudic treatise it stated that the prophet Samuel did write the book of Ruth. As it is does not provide strong evidence concerning the authorship, several authors in recent times have refuted the prophet Samuel as the actual author of this phenomenal book. Does it really matter whether it was written by a male or female author? This has been a question in the minds of many because in its entirety, the content of the book is very positive and has a lot of moral, social and legal coverage, which has provided a lot of insight into the way the Israelites interacted amongst themselves as well as among foreign nations, like the Moabites. In this essay, various perspectives of scholars will be addressed and references to the actual text will also be made to support and refute arguments which have been put forth by various intellectual minds. In a recent book entitled the Widow’s plea, a group of authors cited a number of key points about the book of Ruth. Central to their arguments were factors that provided an insight to the gender identity of the author. Firstly, they referred to a deliberate attempt by the author to strengthen the plight of widowed women in this book, by the avoidance of dealing with the Leveriate marriage, a popular custom that existed during that period. Lawson Younger in made inference to this point by referring to the book ‘Three Shekels’ by H Shanks: The inscription is puzzling. According to the law of levirate marriage, a man must marry his brother’s widow if his brother died childless (see Deuteronomy 25:5–6). Why wasn’t the husband’s brother, who had already received the wheat ? eld in Nacamah, required to do his duty by marrying his brother’s widow? Alas, the ostracon does not tell us(p 32) The laws relating to marriage and redemption were strongly intertwined and since it was not completely applicable in this case because Ruth did not marry her direct next of kin, these scholars have speculated that it might have been authored by a female. Furthermore, H Avolos backed this argument by purporting the deliberate exclusion in this case. (Avolos 616) In an attempt to further prove this point Young referred to various Hebrew texts and phrases used to give a better view point. For instance, the use of the words ‘Amah’ and ‘siphah were used at different times, suggestive of various points regarding the nocturnal influence of Ruth in Chapter 3:9. This is what he noted: Amah seems to be used to emphasize a slave’s feminine qualities (need for protect weakness, sexual attractiveness, etc. ), while siphah seems to be used when the female is viewed as a possession and a laborer. 41 Both terms can be used as self-designations. When it is used this way, amah appears to suggest a female petitioner’s weakness and need for help or protection when presenting a request before a more powerful male, never before another female. When siphah is used as a self-designation of obeisance, it seems to signify the woman’s subservience and readiness to serve or obey instructions. (p127) With the usage of the term amah, despite the fact that she was a labourer or indirectly in servitude, she was still able to realize her feminity even in hardship. It was suggestive of her need for protection and dependency on the one who is stronger than herself, especially when requesting a favour from an influential male figure. This point alludes to the kind of writing by a male author, realizing the need of the woman for protection and a bulwark. The usage of the term siphah suggested the readiness of the woman to adhere to instructions from the male and obey and to do is bidding. In both cases, there is a strong suggestion of a male authorship based on this viewpoint. Further Hebrew terms used indicate more usage of feminine forms of verbs. For instance, the word hyrja, meaning ‘them’ was used to refer to bodies of individuals. Its frequent usage indicated that the author made reference to a wide range of people who were female. Perhaps this could have been suggestive of a male author appreciating the role of the women or in actual fact, a woman who sought to enhance the role of the female in that era? The Feminist Companion of the Bible addressed several points that alluded to an obvious female authorship. (pg 34) It was argued that this book presented various points which gave credence to the importance of women in the Holy Bible. For instance, several women were identified who played excellent roles in biblical event, including Deborah, Dorcas, Esther and Miriam just to mention a few. It was also admitted that there were also major indications to the book of Ruth being authored by a male because of its superscription, just as the case was in other books like the book of Ecclesiastes. An interesting point to note was that there was a chance that although it may not have been written by a woman, the male author certainly had a profound respect for women and valued their contribution to the society. Interestingly, this book under the ‘Unconventional Life Partnership, Women Do Go Unconventional Ways’ heading also referred to the instruction by Naomi to Ruth, to use her natural female charm to win the heart of Boaz. (Athalya 29). This could very well have been a masculine viewpoint, in the sense that Naomi did recognize that Ruth needed a man in her life and hence was giving her advise on how to make Boaz notice her. During that period, it was generally expected that the women realized that they were being prepared for marriage at some stage and it would have been the role of an experienced woman like Naomi to share this with Ruth. This is a very feminine disposition which could have been expressed by the author. In the second chapter of the book of Ruth, she was portrayed as a hardworking lady who went to ‘glean with the reapers’. In Hebrew, the word ‘reapers’ refers to masculinity. Hence, irrespective of this fact, she was able to go and work with the men, gathering enough food supply for herself and for Naomi. As a matter of fact, it was emphasized that she worked through to the period of harvest. By so doing, she was able to gather more than sufficient rations to tend to the needs of a whole family. This was a progressive woman who knew that it was essential that she managed the home front successfully. In other words, she was a traditional homemaker – the major role of the woman. A female author would not have put this point any better. In his comparative study of the book of Ruth, Younger suggested that to a large extent, it seemed that a woman’s economic well-being was directly related to her link with some male. (p 129) From a feminine perspective it could be argued that this exhibited self-reliance and independence of the woman. On the contrary, there is also the aspect of the masculinity portrayed. The head of the reaper, when approached by Boaz who had not yet met Ruth at the time to ask about who she was, the reaper made excuses on her behalf, as she was a foreigner ( a Moabite girl) who did not the customs and modest requirements of the women of Israel. This impled that she was gleaning with the wrong set of people, in other words, the men. Hence, when Boaz eventually spoke to her, he advised her to glean with the girls or young ladies (Chapter 2:8). This was indicative of the male dominance and belief in the distinct and separate roles of the woman and the man. The geneological account in Chapter Four included women in it and this has made people suggest its female authorship. It was uncommon to have women included in the genelogical chronology. Especially in this instant, this geneology led all the way down to David, from whom the expected lineage of the Messiah was from. The character of Boaz was one of a generous and extremely loving individual. He saw the needs of Ruth and Naomi and took the necessary steps to ensure their happiness. By so doing, he tremendously brought a lot of benefits to himself. Eventually, he got married to Ruth. His numerous character traits are worthy of further study. Ruth was described as a virtuous woman. From the beginning of the book, she displayed loyalty and love, even to its end. When Naomi was bereaved of three members of her family including her husband and sons, Ruth did not seek out her own gain but decided to remain with Naomi, always. It took superhuman power to make such a profound statement in Chapter 1:16, entreating Naomi to let her (Ruth) remain with her. There was nothing that Naomi had to offer her. Both her sons were now dead, including the husband of Ruth. As was pointed out by Naomi, she had nothing to offer Ruth. Notwithstanding, Ruth made a selfless commitment to stand by Ruth, through times of sorrow and happiness. Naomi was a wise lady whose experience was of great benefit to Boaz and Ruth. In essence, she took the practical steps to facilitate their union. In all three characters, excellent themes of relational interactions have been displayed. Humility, sacrificial love, generousity and empathy just to mention a few, are required in everyday dealings with people. Regardless of the authorship, these character traits spread across boundaries and are pertinent in dealing with practical real-life matters. Especially today, these attitudes are still required and go along way in boosting human interactions. Works Cited Brenner, A. The feminist companion to the Bible (Second Series) Sheffield Academic Press Avalos, H. Legal and Social Institutions, 616 Malick, D. An Argument of the Book of Ruth from http://www. bible. org/page. php? page_id=952 Shanks, H. (1997). Three Shekels pg 32. Younger, K. L. (1998). Two Comparative Notes on the Book of Ruth. Trinity International University

Womens Clothing Industry Report Essay Example for Free

Womens Clothing Industry Report Essay This industry includes establishments primarily engaged in the retail sale of a general line of womens ready-to-wear clothing. This category also includes establishments primarily engaged in the specialized retail sale of womens coats, suits, and dresses. Custom tailors primarily engaged in making womens clothing to individual order are classified in SIC 5699: Miscellaneous Apparel and Accessory Stores. Industry Snapshot In 2005, $36.7 billion was spent at stores devoted exclusively to womens apparel, a jump of more than $2 billion from 2004 and more than $4 billion from 2003. While the discounters, namely Target and Wal-Mart, were continuing to do well, the more upscale stores were seeing a new surge in spending. While consumer demand for luxury and high quality items was high, most of the purchases in this category were made for those items either from lower-priced brands or items on sale. In the mid-2000s, those employed by the industry were mostly working as sales associates, who are responsible for performing customer service and a variety of operational duties such as setting up displays and organizing stock. Store managers oversee sales, operations, and personnel functions. Merchandisers work with the apparel manufacturers to select apparel for the retailer and control merchandise expenses. According to the U.S. Census Bureau, womens clothing stores reported combined sales of $38.5 billion in 2008. As the economy continued to struggle sales fell 7.6 percent to $35.6 billion in 2009. As sales declined, Womens clothing stores were adapting to the downturn by focusing on recession-friendly prices, and opening up more discount outlets. In fact, there were 345 outlet stores slated to open in 2011, according to the November 2009 Retail Forward report. Organization and Structure The structure of the U.S. retail industry, including womens clothing stores, has changed significantly since the early 1990s, moving from a production-driven market to a consumer-driven market. Nontraditional retailers, such as discounters, off-priced stores, and factory outlets, fared well. Because of continuing competition from nontraditional retailers, department stores such as J.C. Penney and specialty stores such as The  Limited increased their focus on private labels. In the mid-1990s, consumers demanded more convenience and quicker service from growing no-store retailing, particularly in direct-mail order, television, and online shopping. An Internet shopping study by Ernst Young LLP reported that the number of retailers selling online tripled in 1998 to 39 percent. The online market was estimated to reach $13 billion in sales at the end of 1999. The relationship between larger retailers and suppliers significantly intensified because a growing number of retailers were taking on entrepreneurial roles traditionally performed by apparel producers. Larger retailers and direct-mail order companies were making decisions in areas such as product design, fabric selection and procurement, and apparel production, which in turn influenced production scheduling, pricing, and delivery dates. Background and Development Womens clothing stores were introduced in Europe in the late 1700sslightly later in the American coloniesat a time when productive capability, population, and prosperity allowed clothing production to move out of the house and into the factory, and clothes to move into retail stores. Around this time, seamstresses began opening shops offering custom-made hats, dresses, cloaks, or other garments. These garments of the latest fashion were for those who could afford to hire out the work of stitching. Trading posts in the frontier areas carried cloth and some ready-made apparel. The invention of the sewing machine, the rise of mass production, and the proliferation of retail stores by the late nineteenth century led people first to sample and later to rely on ready-made clothing for sale as a reliable means of obtaining fashionable clothing. In the 1890s, ready-to-wear clothing came into its own, and by the turn of the century ready-made womens wear was available in abundance in the Unit ed States. By the 1920s, it was considered more fashionable to buy clothing from a store than to make it at home. For many years, the department store and the downtown womens shop were the mainstays of womens wear retailing. Department stores offering a vast selection of goods and specialty stores catering to unique tastes dotted the urban landscape. For those with enough money, shopping became a social event. Along with the growth of womens clothing retailing came the increasing importance of fashion. The womens  apparel industry established a voice in government through the National Retail Federation (NRF), the trade group representing the entire spectrum of the nations retail industry. In the early and mid-1990s, the NRF lobbied the U.S. Congress on issues such as minimum wages and the proposed health care plan. The NRF was opposed to an increase in the minimum wage on the grounds that many retailers would have to close down operations or fire staff to meet expenses with a higher wage base. In 1994, Womens Wear Daily reported that the NRF opposed the Clinton administrations proposed univers al health coverage on the grounds that more than 700,000 jobs would have to be eliminated in all retailing. At that time only 35 percent of retail workers received health care benefits. The NRF supported a plan that emphasized offering health coverage but did not require employers to pay for that coverage and allowed for the creation of purchasing pools for group insurance. Heading into the twenty-first century, dedicated womens stores faced renewed competition from alternative retail venues offering specialty or general line womens apparel in addition to other product lines. Sporting goods retailers were devising new strategies to increase womens apparel business. In 1995, womens apparel ranged from 10 percent to 40 percent of store merchandise. Sporting goods retailers saw strong potential in the womens apparel market. Retailers increased floor space to accommodate womens products; set up womens departments; increased stock of best-selling brands; and held store events to draw more female customers. Department stores also responded to the increased demand for womens apparel and began rep ositioning themselves to win back the customers they had lost to more focused outlets like The Gap and The Limited. Such retailers as Bloomingdales and Dayton Hudson revamped the womens apparel collections. The large-sized womens clothing market grabbed the attention of clothing retailers in the mid-1990s with sales reaching $20 billion and claiming 24.7 percent of the market. The key factors that influenced these sales were an increase in fashions featuring younger silhouettes and the use of better fabrics. Lane Bryant, a division of The Limited Inc., brought in more fashionable clothes and worked to change the perception of large-size fashion. Our customer wants to wear the exact same fashion her skinny friends wear, noted Lane Bryants chief executive Jill Dean in a 1999 Wall Street Journal interview. One of the hottest growth areas in retailing  during the late 1990s was discounting. Clothing retailers saw an opportunity to bring fashionable clothes at reasonable prices to the masses. In 2002, Target was the countrys third-largest discounter and a $40 billion division of the Dayton Hudson Cor poration. Nearly 35 percent of Targets sales come from the clothing department. Old Navy, a division of The Gap, was launched in 1994 to compete with stores like Sears and Target with this concept in mind. As the United States initiated the war with Iraq in March of 2003, the U.S. economy remained soft and consumers remained cautious. As a result, the clothing industry reported sales numbers below those previously forecasted, down 6 to 7 percent rather than the anticipated 3 to 4 percent for the month. The retailers with the most successor least amount of declinewere those that offered moderate-priced, affordable sportswear that combined the right amount of fashion with value. By the end of 2003, the industry saw increased spending again, a trend that continued into 2004. Dresses, skirts, and tailored clothing all declined in overall sales and units sold, but increases were seen in lingerie, suits, swimwear, and knit shirts. Tops were hailed as the new accessory in 2005, and women were being bolder about choices in this division, while preferring more versatile, classic apparel items in other categories such as slacks. The so-called career/casual market was on the upswing, as women looked for clothing that could easily make the transition from work to leisure. While fashion was important in the mid-2000s, so was the desire to simplify. Although higher-end, upscale items were increasing in demand, women still looked for the bargain, leading to an increase in the affordable luxury category as well. While overall spending was up, the average price per item was down. Retailers also continued to target junior shoppers as a consistent source for revenues. Young consumers, who tend to have more disposable income than older shoppers, spend more money on clothing and are more conscious of fashion trends. In 2007, those junior shoppers were interested in buying dresses, as were most other women. According to a report by The NPD Group, revenues from sales of dresses increased 30.4 percent overall for the 12-month period ending in April 2007. For juniors, the jump was 53.3 percent, while the sales of misses dresses rose 33.1 percent and the sale of petite dresses 31.6 percent. During that 12-month period, sales of womens apparel in general increased 5.1 percent. Even with positive signs  in sales heading into the late 2000s, an uncertain economy had one of the stronger chains slimming down to improve profitability. Despite net sales that rose 2.3 percent in 2007 to approximately $2.4 billion, Ann Taylor Stores Corporation announced in January 2008 that it planned to close 117 stores from 2008 through 2010 as a restructuring of the business. Current Conditions Retailers are crafting marketing and merchandising campaigns around the new normal, making a bid for female shoppers still shell-shocked by the biggest economic downturn since The Great Depression. Consequently, consumer spending patterns have been changing and will likely continue to evolve. The average consumer was shifting to fewer purchases. One market research firm that follows the industry reported sales of womens clothing fell 2.8 percent in 2008 and 4.9 percent in 2009. Industry watchers dont see the downward trend changing anytime soon, especially since income levels were on the decline and the unemployment rate remained at high levels and apparel in general being a discretionary purchase was suffering because of the weak economy. In one survey conducted by ShopperScape by Ohio-based Retail Forward based on all age groups and income levels found over half of women shoppers will replace only their worn out clothing. Additionally, when it comes to buying clothing, shoes, and accessories the mentality was to trade down selecting the less expensive brands. Additionally, women shoppers were much more value oriented then in years past. The mentality to shop till you drop was now labeled frugal shopper seeking out the less expensive designer replicas. Some womens clothing stores underwent significant restructuring downsizing throughout the late 2000s. One industry leader, Ann Taylor Stores including their LOFT stores shed 60 stores in 2008, followed by another 42 stores in 2009 with about another 72 announced to close in 2010. Of the estimated 174 store closures, half would be Ann Taylor Stores and about half would be LOFT stores. Industry Leaders Some of the leaders in the womens clothing store retail industry in the United States were The Gap, Inc., Limited Brands, Inc., Charming Shoppes, Inc., Talbots, Inc., Ann Taylor Stores Corporation, and the Dress Barn, Inc. The Gap, founded in 1969 by Don and Doris Fisher in San Francisco, has  become an international specialty retailer offering mens, womens, and childrens casual clothing and accessories. The Gap operated thousands of stores in six countries, including The Gap, GapKids, Baby Gap, GapBody, Banana Republic, and Old Navy Clothing Co. The Gap has stores in the United States, Japan, the United Kingdom, Canada, France, and Germany. The company reported $15.9 billion in revenues for 2007. The Gap expanded quickly in the 1980s, purchasing the Banana Republic chain in 1983, launching GapKids, and BabyGap in 1986, and opening its first overseas store in London in 1987. By 1990 The Gap was one of the most successful apparel retailers and the second largest clothing brand in th e United States. One of the biggest successes for The Gap was the Old Navy division, launched in 1994. In less than three years, The Gap opened 282 Old Navy stores and hit sales of $1 billion. Gap Online was introduced in November 1997. Limited Brands, Inc., the top U.S. womens apparel retailer, was founded in 1963. Limited Brands, Inc. shed its Express and The Limited apparel chains to focus on its Victorias Secret and Bath Body Works stores. The company operated 2,900 specialty stores in North America in 2007. Sales for 2007 reached $10.7 billion. The leader in plus size apparel, Charming Shoppes, Inc., had Fashion Bug and Catherines Plus Size stores, and had acquired Lane Bryant from Limited Brands in 2001. The company reported 2007 sales of $3.1 billion. Talbots, Inc., with nearly 1,380 stores carrying traditional clothing and accessories, reported $2.2 billion in 2007 sales. Ann Taylor Stores, which catered to customers looking for upscale, classic clothing, reported 2007 sales of $2.4 billion. The Dress Barn catered to the professional woman on a budget. The company had $1.3 billion in 2007 sales. With 2,100 stores globally, The Gap reported revenues of $15.7 billion in 2008, falling to $14.1 billion in 2010 with 135,000 employees. Limited Brands, Inc. also saw their revenues decline fro $10.1 billion in 2008 to $8.6 billion in 2010 with 92,100 employees. From a reported $3 billion in sales for 2008, Charming Shoppes, Inc.s revenue plummeted to nearly $2.1 billion in 2010 with 27,000 employees. Talbots, Inc.s total number of stores fell from 1,380 in 2007 to 580 in 2010, as did its revenues from nearly $2.3 billion in 2008 to $1.2 billion in 2010 with 9,100 employees. Ann Taylor Stores reported revenues of $1.8 billion in 2010, well below the reported revenues of nearly $1.4 billion in 2008. The company employed  18,800 people. The Dress barn reported revenues of $982 million in 2010.

Immune Responses of Cytokine Adjuvented DNA Vaccine

Immune Responses of Cytokine Adjuvented DNA Vaccine KOTLA SIVAREDDY Title for the Ph.D Thesis â€Å"Study on Immune responses of Cytokine Adjuvented DNA vaccine (genes coding for structural proteins) for FMD delivered by cationic PLG micro particles†. Objectives: To clone gene coding for structural protein of FMDV in eukaryotic expression vector (pC DNA) under CMV promoter. To clone bovine interleukin 18 gene in suitable cloning vector and express the same in Bacterial/yeast expression system for characterization of expressed recombinant protein. To construct a vaccine construct consisting of FMDV P1 (structural), 3C (Viral protease), and Bovine interleukin 18 genes in eukaryotic expression system under CMV promoter (pC DNA). To study the expression of the vaccine constructs in vitro in BHK-21cells. PLG micro particles’ preparation and characterization. To study the immune responses of the DNA vaccine in experimental animals (guinea pigs / cattle). Foot-and-mouth disease (FMD) caused by foot-and mouth disease virus (FMDV) is an infectious disease affecting cloven-hoofed animals, and poses a serious threat for animal health and exacts an economic toll on the livestock industry. FMD viral genome is a positive-sense single stranded RNA of approximately 8.5kb. The viral RNA genome is translated as a single polypeptide precursor that is subsequently processed by virus-encoded proteases 2A and 3C to produce the structural and non-structural proteins required for virus assembly and replication. One of the initial polypeptide cleavages, mediated by the 2A protein, is a co-translational cleavage at its own C terminus to release it from the 2B protein. The viral 3C proteinase subsequently processes the structural protein precursor, P1-2A, into the capsid proteins, VP0, VP3, and VP1, and the non structural peptide, 2A. These proteins then self assemble to form empty icosahedral capsid particles that contain 60 copies of each protein. Immu nological studies have identified linear and conformational sites that are present on both empty capsids and virions, and antiserum raised against either form has the same serological specificity. Thus, the structural protein precursor, P1-2A, and the 3C protease of FMDV are desirable immune antigens for new vaccine development. In countries where disease eradication has not been achieved, vaccination plays a crucial role in its control. Although inactivated virus vaccines effectively prevent FMD, they have several limitations like short duration of immunity, incomplete viral inactivation and virus escape from vaccine producing facilities. As a result, alternative approaches are being investigated, including the construction of modified live virus, subunit vaccines, synthetic peptides, naked DNA plasmids. DNA vaccination which offers several promising features i.e., DNA is convenient to manufacture and store, its production is safe, sequences from circulating strains can be easily i ncorporated in the vaccines, and it also allows the discrimination of the infected from the vaccinated animals. Several reports have shown the efficiency of DNA vaccination to induce protective immunity in the mouse model. However, the primary difficulty with DNA vaccination is its poor immunogenicity in target species. Cytokines are being used as molecular adjuvants by co administering with DNA vaccines to improve the efficacy of the vaccine. Cytokines play an important role both in the development of a functional immune system as well as in the responses of the organism to infection. Interleukin18 (IL18) is a potent interferon ÃŽ ³ (IFNÃŽ ³) inducing factor (IGIF), enhances Th1 immune responses. Recent studies have shown that IL18 also promote Th-2 type responses and increases dendritic cell (DC) number in lymph nodes in mice. In addition, IL18 has been used as an adjuvant to DNA vaccines for classical swine fever virus, pseudo rabies virus, porcine reproductive and respiratory sy ndrome virus.IL18 was co expressed along with FMDV VP1 in Pichia as fusion protein has enhanced humoral responses and marginally the CMI response in mice. Recombinant fowl pox co-expressing FMDV P1 2A3C and IL18 enhanced the immune responses and gave higher protection in swine Many other studies have shown the positive effect of plasmid encoding the IL-18 as a molecular adjuvant on DNA vaccinations. Efficacy of DNA vaccine could be improved by the inclusion of adjuvants and good vaccine delivery systems. Importantly, cationic microparticle with adsorbed DNA induced enhanced immune responses in comparison to naked DNA and this enhancement was apparent in all species evaluated, including nonhuman primates. Cationic PLG microparticles appear to be effective predominantly as a consequence of the efficient delivery of the adsorbed DNA into DC. Following administration, the micro particles are also very effective at recruiting DC to the injection site, and the micro particles also protect adsorbed DNA against degradation in vivo. A second useful property of micro particles is that they can present multiple copies of antigens on their surface, which has been shown to be optimal for B cell activation. The main advantage of this type of association is the efficient immobilisation of plasmid DNA on the microparticle surface without compromising its integrity. Moreover, after administration, the release of cationic DNA complexes from the surface appeared to facilitate the transfection of cells .At this point, it is not completely understood, whether the adsorption on cationic micro particles can protect plasmid DNA from cleavage through endonucleases after administration in vivo. Nevertheless, release of cationic DNA complexes is expected to provide better protection as compared to release of free DNA. Beside their inherent safety and ease of administration, they improve the DNA capture by antigen presenting cells (APC) and stimulate APC maturation. DNA delivery via PLG has been successfully used to vaccinate against several infections in mice, guinea pigs and even in macaques models. 1. AMPLIFICATION and cloning of gene sequence coding for P12A 3C FMDV serotype ‘Asia1’ gene coding for the polyprotein, P1-2A (2.3 kb) was amplified from viral genome, of the serotype ‘Asia1’ polyprotein gene, using VP4L (Bac) and 2AR (E.coR1) primers. Cloned in to pC DNA at E.co R I, BamHI sites. Ligated and transformed in to DH alpha 5 cells. Transformants are screened by colony PCR by using insert specific primers. Orientation was checked by PCR. Insert release was confirmed by RE digestion by using E.co R I, Bam HI. 3C coding sequences were amplified from clone available in lab. 0.6 Kb was amplified .The purified amplicon was digested and ligated in to p C DNA and transferred in to competent DH5à ¯Ã‚ Ã‚ ¡ cells upon screening by PCR and by re digestion positive clones were conformed. 2. Cytokine amplification (IL18) and cloning in prokaryotic / yeast pC DNA expression vector and characterization of expressed protein. Interleukin 18 (IL18) modulates immune functions by inducing interferon–Î ³(IFN-ÃŽ ³) production and promoting Th1 immune responses. In the present study I amplified and cloned the sequence (582 bp) encoding full length bovine IL18 from peripheral blood mononuclear cells (PBMC) stimulated with Phytohaemoglutinin (PHA). Nucleotide and the deduced amino acid sequence of the cloned IL18 showed an identity of 86-98% with IL 18 sequences of the other ruminants compared. The insert was sub cloned in to eukaryotic expression vector (PcDNA) .The specificity of the expressed IL 18 was confirmed by western blotting. The insert was sub cloned in to pET 32a vector and expressed in E.Coli as fusion protein of 42kDa. The specificity of the expressed IL 18 was confirmed by western blotting. The biological activity of the purified protein was analysed for its ability to induce IFN-ÃŽ ³ production in PBMC as measured by Enzyme linked immunosorbent assay (ELISA) and quantitative polymerase cha in reaction (qPCR). IL18 anti FMD viral activity was conformed in vitro in BHK-21 cells by using plaque assay; viral replication was quantified by Real time PCR, ELISA and titration assays. 3. Study of the expression of the constructs in vitro in BHK-21 Cells Expression of cloned P12A3C and IL18 genes were studied in mammalian expression system for confirming the frame and intactness. The P12A3C, IL18 genes cloned under Eukaryotic promoter was transfected in BHK 21 cells with lipid based lipofectamine. Subsequently, the proteins were confirmed by Western blotting by using using anti FMDV serotype ‘Asia’, serum from experimentally infected cattle. IL18 transfected cell lysate showed 18 KDa by using human IL18 Mab. 4. PLG microparticles preparation and characterization The PLG/CTAB micro particles were prepared using a solvent evaporation technique essentially as described previously and briefly, the micro particles were prepared by emulsifying 10ml of a 6% (w/v) polymer solution in methylene chloride with 1ml of TE buffer at high speed using an soniprep. The primary emulsion was then added to 50ml of distilled water containing CTAB (0.5%, w/v). This resulted in the formation of a water/oil/water emulsion which was stirred at 6000rpm for 12h at room temperature, allowing the methylene chloride to evaporate. The resultingmicro particles were washed in distilled water by centrifugation at 10,000 Ãâ€" g and freeze dried. The plasmid construct was adsorbed onto the microparticles by incubating 100 mg of cationic microparticles with 100 mgs (1 mg/ml solution) of plasmid DNA at 40C for 6 h. The coated microparticles were then separated washed with TE and freeze-dried. Amount of plasmid adsorbed on PLG particles was quantified by eluting the DNA by 0.2 N NaOH (incubation for 10 h at 4 0C and measuring the Optical Density (OD) at 260 nm. Blank PLG micro particles controls were run simultaneously to deduct background value. The size distribution of the micro particles was determined using a particle size analyzer and electron microscopy. 5.A. Evaluation of the Immunological response of various DNA vaccine constructs in guinea pigs. Foot and Mouth Disease (FMD) can be controlled by regular vaccination and restricting the movement of animals infected in the endemic countries.. DNA vaccine construct was made with P1-2A3C coding sequences of serotype Asia1 in p C DNA. To evaluate the optimal dose of the construct in guinea pigs, the plasmid was coated on cationic Poly Lacto-co-Glycolide (PLG) micro particles was injected in to guinea pigs at 2,5,10,15,20,30 ug doses intramuscularly. Sera samples collected from the vaccinated animals at 21st dpv were evaluated for immune response by Enzyme linked immunosorbent assay (ELISA), Serum neutralization test (SNT) and MTT assay. Maximum ELISA / SNT titers and MTT stimulation indices were observed at 10  µg dose which also gave 83% protection when the guinea pigs were challenged with homologues virus. 10ug was found to be the optimal dose to guinea pigs. P12A3CpCDNA and bovine IL-18 pcDNA plasmids were constructed under CMV promoter and the coated with Cationic PLG microparticle, immune response of the co administered constructs was evaluated in guinea pigs. Both the plasmids constructed under CMV promoter and 10 µgs each of the plasmids were inoculated intra muscularly in guinea pigs with a booster dose at 21st day post vaccination (dpv). Both humoral and cellular immune response were analysed by IgG1, IgG2 enzyme linked immunosorbent assay (ELISA), Serum neutralization test (SNT) and MTT assay. Th1, Th2 cytokine profile was analysed by real time PCR and the phenotyping of T cell sub population in the peripheral blood was performed by flowcytometry. The results have sown significantly higher humoral and cell mediated immune responses in P12A3CIL18+PLG group than P12A3C IL18, and inactivated virus vaccine inoculated groups. Similarly, higher CD4, CD8 population and Th1, Th2 cytokine levels were seen in former group. P12A3CIL18+PLG vaccine protected all the six animals when challenged with homologous virus compared to five in inactivated virus vaccine group respectively. These results have shown that the plasmid encoding for P12A3C pcDNA when co inoculated with IL18 and PLG induce higher and protective immune responses, suggesting rBoIL-18 and Micro particles has a potential to enhance the efficacy of vaccine against FMD. 5. B Evaluation of the Immunological response of various DNA vaccine constructs in Cattle. Healthy male cattle calves of local breed ( Hallikar Breed) of 6 months to one year age group were purchased from local village shandy( cattle market). These animals were housed in healthy animal shed facilities available at IVRI Animal experimental station at Yelahanka , Bangalore. After initial quarantine the animals were bled and the sera were screened for FMDV antibodies for serotype Asia 1 by SNT. The FMD antibody free animals were divided in to 6 groups of six animals each namely Group I to Group IV. All the group were vaccinated with each construct with 200 ug injected by intramuscularly except conventional vaccine group injected with 2 ml of FMDV Inactivated vaccine. One group kepted for control group (vaccinated with PBS) .After 21 st days of first vaccination with same amount booster dose was injected P12A3CpCDNA and bovine IL-18 pcDNA plasmids were constructed under CMV promoter and the coated with Cationic PLG microparticle, immune response of the co administered constructs was evaluated in guinea pigs. Both the plasmids constructed under CMV promoter and 200 µgs each of the plasmids were inoculated intra muscularly in calves with a booster dose at 21st day post vaccination (dpv). Both humoral and cellular immune response were analysed by IgG1, IgG2 enzyme linked immunosorbent assay (ELISA), Serum neutralization test (SNT) and MTT assay. Th1, Th2 cytokine profile was analysed by real time PCR (ÃŽ ³IFN, IL4, IL2, ÃŽ ±IFN, IL12, IL25,TLR-4,TLR3,TLR-2,IL8,IL10) and the phenotyping of T cell sub population (CD4 and CD8) and intracellular cytokine molecules (ÃŽ ³IFN, IL4, IL2) in the peripheral blood was performed by flowcytometry. The results have sown significantly higher humoral and cell mediated immune responses in P12A3CIL18+PLG group than P12A3C IL18, and inactivated virus va ccine inoculated groups. Similarly, higher CD4, CD8 population and Th1, Th2 cytokine levels were seen in former group. P12A3CIL18+PLG vaccine protected four out of six animals when challenged with homologous virus compared to 3 in inactivated virus vaccine group respectively. Non structural proteins,ELISA conformed in challenged animals.These results have shown that the plasmid encoding for P12A3C pcDNA when co inoculated with IL18 and PLG induce higher and protective immune responses, suggesting rBoIL-18 and Micro particles has a potential to enhance the efficacy of vaccine against FMD Journal papers and conference/seminar papers from Doctoral research work 1. Expression of Bovine (Bos indicus) interleukin-18 inEscherichia coli and its biological activity.Kotla Siva Reddy, Dowlathabad. Muralidhar Rao, Hosur Joyappa Dechamma,Veluvarthy V.S. Suryanarayana and Golla Ramalinga Reddy.Published in Microbiology and Immunology 2010; 54: 564–567. 2. Enhancement of DNA vaccine (P12A3C-pcDNA) efficacy against Foot- andMouth Disease by co-administration of Interleukin-18 expressing (IL18pcDNA) plasmid in Guinea Pigs. Siva Reddy .K. Muralidhar Rao.D., Badrinaryana.M. Suryanaryana.VVS. and Reddy G.R. Accepted in FEMS Immunology and Medical Microbiology. Dec -2010 1–9. 3. Dose optimization of Cationic PLG micro particle coated DNA vaccine against Foot and Mouth Disease in Guinea pigs. Siva Reddy, K.,Rashmi., B.R., Muralidhar Rao, D., Dechamma H.J., Banumathi .N., Suryanarayana V.V.S and Reddy .G.R. accepted in J.of Life science.(Article in press) 4. Cytokine profile studied by Real time PCR in FMDV antigen stimulated Bovine PBMC cells. Siva Reddy .K., Muralidhar Rao,D.,PrabhuDas,K., Suryanaryana.VVS., Reddy ,G.R. Accepted in Journal of Biotechnology , Bio engineering and Bio –Informatics. (Article in press). 5. Bos indicus Interleukin 18 complete coding sequence published in NCBI Gen bank .SivaReddy,K., Muralidhar Rao,D., Dechamma,H., Banumathi,N.,Suryanaryana,V. and Reddy,G. Acc.No. FJ985771 6. Enhancement of DNA vaccine (P12A3C-pcDNA) efficacy against Foot- andMouth Disease by co-administration of Interleukin-18 expressing (IL18pcDNA) plasmid in Guinea Pigs. Siva Reddy .K. Muralidhar Rao.D., Badrinaryana.M. Suryanaryana.VVS. and Reddy G.R. Presented in Society for applied biotech biotechnology (SAB) annual conference at Dharmapuri Dec 17,18 7.Bovine Interleukin -18 inhibits Foot-and-Mouth Disease virus Replication in BHK- 21 cells. K. Siva Reddy, D.Murali Dhar Rao, Kakoli Ahmed, H.J Dechamma N.Bhanumathi ,VVS Suryanarayana ,G.R Reddy presented at VIROCON 2010 XIX National Conference RECENT TRENDS IN VIRAL DISEASE PROBLEMS AND MANAGEMENT SVU Tirupathi, Mar 18-20 ,2010 . 8. Cationic Micro Particle (PLG) coated DNA vaccination Induces a long term immune response and Protective Immunity against Foot –and-Mouth disease virus. K. Siva Reddy, Rashmi Dechamma N.Bhanumathi ,VVS Suryanarayana ,G.R Reddy Presented at VIROCON 2010 XIX National Conference RECENT TRENDS IN VIRAL DISEASE PROBLEMS AND MANAGEMENT SVU Tirupathi, Mar 18-20 ,2010. 9. Dose response studies of ID- p VAC (SECRETORY VECTOR CONSTRUCT) coated on cationic PLG micro particles against FMDV in guinea pigs. Siva Reddy K., Reddy G.R. Presented at SBC Annual conference Impact of Basic and Translational Research on Medicine, Agriculture and Industry, IIT Madras 18-20 DEC -2008. Communicated Articles 1. Cationic Micro Particle (PLG) coated DNA vaccination induces a long term immune response and Protective Immunity against FMD in GuineaPigs. Siva Reddy ,K ., MuraliDhar Rao,D.,Rashmi, B.R., Dechamma H,J., Banumathi.,N., Suryanarayana V.V.S and Reddy G.R Communicated in to Vet Immunology and Immunopathology(Under review). 2. Bovine Interleukin 18 inhibits Foot and mouth disease virus replication in BHK-21 Cells. K. Siva Reddy, D.MuraliDhar Rao, K.PrabhuDas, VVS Suryanarayana,G.R Reddy communicated in to Biotechnology and Applied Biochemistry. 3. Enhanced efficacy of a Foot and mouth disease DNA vaccine (P12A3CpcDNA) by adsorption onto cationic PLG microparticle in guinea pigs .K. Siva Reddy, D.MuraliDhar Rao, K.PrabhuDas, VVS Suryanarayana ,G.R Reddy communicated into International journal of Immunopharmacology.

Essay --

To the farmer, soil is where crops grow. To the engineer, soil is a foundation upon which to build. To the ecologist, soil supports communities of living things. To the archaeologist, soil holds clues to past cultures. To the city dweller, soil nurtures grass and gardens. To the scientist, soil is all of these things. Soil has been called â€Å"the skin of the earth† because it is the thin outermost layer of the Earth’s crust. Like our own skin, we can’t live without soil (â€Å"Story of Soil†). No matter where we go or what we do, soil is important to all of us. We walk all over it, take it for granted, and treat it like dirt, but SOIL is what our lives depend on. Every single thing we do is rooted in soil in at least one way, if not more. If we dig deep enough, we may even find our future careers and life passions. There are countless career opportunities that rely on soil in one way or another. What soil means to each career pathway varies greatly, much like the diversity within the career options themselves. No matter how different our lives and careers may be, one thing is for certain: we absolutely cannot survive without soil. To the farmer, soil is vital to his very existence. Soil is the home of over 400 million acres of crops that the farmer grows and nurtures to feed his family and yours. According to the 2007 USDA Census, there are almost 1 billion acres of farmland being utilized for crops and livestock (â€Å"American Farms†). Without the soil, these farmers would not be able to raise the products that we rely on for basic survival. United States agriculture must produce enough food to help feed the world’s ever growing population. At 7 billion people and rising, our soil must remain as productive as ever (â€Å"World Population Clock... ...bringing wetland habitats back and creating better soil management practices. Even if ones’ career is not directly related to soil, we all rely on it every day. To urban dwellers and rural route residents, soil supplies everything needed for their survival. Soil supplies the antibiotics that fight disease, the food on our plates, the clothes on our backs and acts as a holding facility for solid waste and wastewater. Soil also provides the foundation for our homes, stores, schools, and jobs. This is just the tip of the iceberg describing how our existence and possibly even our careers rely on soil. As we can see, a variety of diverse jobs include working with and understanding soil. Soil is vital to the farmer, to the archaeologist, to the scientist, to the ecologist, to the architect, to the engineer, to you and to me. Like our own skin, we can’t live without soil. Essay -- To the farmer, soil is where crops grow. To the engineer, soil is a foundation upon which to build. To the ecologist, soil supports communities of living things. To the archaeologist, soil holds clues to past cultures. To the city dweller, soil nurtures grass and gardens. To the scientist, soil is all of these things. Soil has been called â€Å"the skin of the earth† because it is the thin outermost layer of the Earth’s crust. Like our own skin, we can’t live without soil (â€Å"Story of Soil†). No matter where we go or what we do, soil is important to all of us. We walk all over it, take it for granted, and treat it like dirt, but SOIL is what our lives depend on. Every single thing we do is rooted in soil in at least one way, if not more. If we dig deep enough, we may even find our future careers and life passions. There are countless career opportunities that rely on soil in one way or another. What soil means to each career pathway varies greatly, much like the diversity within the career options themselves. No matter how different our lives and careers may be, one thing is for certain: we absolutely cannot survive without soil. To the farmer, soil is vital to his very existence. Soil is the home of over 400 million acres of crops that the farmer grows and nurtures to feed his family and yours. According to the 2007 USDA Census, there are almost 1 billion acres of farmland being utilized for crops and livestock (â€Å"American Farms†). Without the soil, these farmers would not be able to raise the products that we rely on for basic survival. United States agriculture must produce enough food to help feed the world’s ever growing population. At 7 billion people and rising, our soil must remain as productive as ever (â€Å"World Population Clock... ...bringing wetland habitats back and creating better soil management practices. Even if ones’ career is not directly related to soil, we all rely on it every day. To urban dwellers and rural route residents, soil supplies everything needed for their survival. Soil supplies the antibiotics that fight disease, the food on our plates, the clothes on our backs and acts as a holding facility for solid waste and wastewater. Soil also provides the foundation for our homes, stores, schools, and jobs. This is just the tip of the iceberg describing how our existence and possibly even our careers rely on soil. As we can see, a variety of diverse jobs include working with and understanding soil. Soil is vital to the farmer, to the archaeologist, to the scientist, to the ecologist, to the architect, to the engineer, to you and to me. Like our own skin, we can’t live without soil.

Protecting Women from Domestic Violence Essay -- Abuse Violence Essays

â€Å"Domestic violence is the most ubiquitous constant in women’s lives around the world. There is virtually no place where it is not a significant problem, and women of no race, class, or age are exempt from its reach† -Joni Seager Abstract: Domestic violence against women is a social problem that occurs in nearly every corner of the world. Recently, some states have begun to recognize that women must be protected from abuse by family members and intimates. While policies and practices designed to protect women have emerged in a number of countries, many lag behind on the issue. This paper will examine the causal factors behind the variation in protection for women. The literature on women and politics suggests that women’s representation may increase the level of protection against domestic violence because female legislators are more likely to put women’s issues on the agenda and make policy choices that benefit their sex. Alternatively, the culture of a state may determine whether the society supports rights for women, including protection from domestic violence. A variety of statistics indicating women’s representation and culture were gathered for analysis. Using bi variate cor relation and multiple regression, the theories were tested against each other in an attempt to determine the cause of variation in levels of protection. The findings suggest that both women’s representation and culture are significantly correlated to level of protection. Because the number of women in elected office influences protection to a greater extent than culture, improving women’s representation appears to be an important factor in fighting the domestic violence problem. THE DOMESTIC VIOLENCE PROBLEM Across... .... [3] For data source, see UN. The World’s Women 2000: Trends and Statistics. NY, 2000. [4] For data source, see World Development Indicators, 2003 [5] For data source, see UN, The World’s Women 2000: Trends and Statistics. NY, 2000. [6] For data sources, see Kamerman, Neuman, Waldfogel and Brooks-Gunn. â€Å"Social Policies, Family Types and Child Outcomes in Selected OECD Countries.† OECD, 2003. Luxembourg Income Study, 1997; expert opinion [7] For data source, see Seager, Joni. The Penguin Atlas of Women in the World. Penguin Books: New York, 2003. [8] For data source, see Seager, Joni. The Penguin Atlas of Women in the World. Penguin Books: New York, 2003. [9] For data source, see The World Bank Statistics, 2001 [10] For data source, see Seager, Joni. The Penguin Atlas of Women in the World. Penguin Books: New York, 2003. Protecting Women from Domestic Violence Essay -- Abuse Violence Essays â€Å"Domestic violence is the most ubiquitous constant in women’s lives around the world. There is virtually no place where it is not a significant problem, and women of no race, class, or age are exempt from its reach† -Joni Seager Abstract: Domestic violence against women is a social problem that occurs in nearly every corner of the world. Recently, some states have begun to recognize that women must be protected from abuse by family members and intimates. While policies and practices designed to protect women have emerged in a number of countries, many lag behind on the issue. This paper will examine the causal factors behind the variation in protection for women. The literature on women and politics suggests that women’s representation may increase the level of protection against domestic violence because female legislators are more likely to put women’s issues on the agenda and make policy choices that benefit their sex. Alternatively, the culture of a state may determine whether the society supports rights for women, including protection from domestic violence. A variety of statistics indicating women’s representation and culture were gathered for analysis. Using bi variate cor relation and multiple regression, the theories were tested against each other in an attempt to determine the cause of variation in levels of protection. The findings suggest that both women’s representation and culture are significantly correlated to level of protection. Because the number of women in elected office influences protection to a greater extent than culture, improving women’s representation appears to be an important factor in fighting the domestic violence problem. THE DOMESTIC VIOLENCE PROBLEM Across... .... [3] For data source, see UN. The World’s Women 2000: Trends and Statistics. NY, 2000. [4] For data source, see World Development Indicators, 2003 [5] For data source, see UN, The World’s Women 2000: Trends and Statistics. NY, 2000. [6] For data sources, see Kamerman, Neuman, Waldfogel and Brooks-Gunn. â€Å"Social Policies, Family Types and Child Outcomes in Selected OECD Countries.† OECD, 2003. Luxembourg Income Study, 1997; expert opinion [7] For data source, see Seager, Joni. The Penguin Atlas of Women in the World. Penguin Books: New York, 2003. [8] For data source, see Seager, Joni. The Penguin Atlas of Women in the World. Penguin Books: New York, 2003. [9] For data source, see The World Bank Statistics, 2001 [10] For data source, see Seager, Joni. The Penguin Atlas of Women in the World. Penguin Books: New York, 2003.

Ghost Story of College Ghost Lure :: Ghost Stories Urban Legends

College Ghost Lore If one were to take the beltway to I-270 north, about an hour north of Washington, DC one would arrive at a town called Emmitsburg, Maryland. Is the town haunted? I collected several stories from a senior in college who is from Emmitsburg. This senior is a white female. The stories I collected, many of which have to do with the small Catholic college indicate a rich ghost lore in the town. There are several stories that this woman told me concerning her hometown. The first is one that most people who live in the town know. In the mid 1800s, a man by the name Larry was born. Larry was the son of a famous composer and musician and came to teach music at the college. His father wanted Larry to be a musician like himself; however Larry was not as skilled. Larry became popular with the college students who would come to his grocery store where he would sing songs for the pretty girls. In the late 1800s, his father died, and Larry was quite sad. The following Christmas, Larry took his flute and went to the cemetery at Mount Saint Mary’s College to play one of his father’s most famous pieces, â€Å"When the Glory Lit the Midnight Air†. The town folk thought he finally mastered the ability to play the flute to honor his father. So the town folk went up to the gravesite by the Grotto of Our Lady of Lourdes on the campus. The event became a tradition, and La rry would lead the people up to the gravesite each Christmas to play the flute. In the 1920s Larry died. Older residents say that if you listen very carefully on Christmas Eve or Christmas morning you can still hear the ghostly strains of beautiful flute music coming from the cemetery. A little while later, the music is gone, not to be heard again for another year. Another story the storyteller told me is that about Father Brute. One of the earliest presidents of the school was Reverend Simon Brute. He died in the mid 1800s. Brute still glides about the campus wearing long black robes. People who have seen his ghost describe the same sunken cheeks and other particular features. He usually smiles and nods and moves on. In Brute Hall is the room that Brute once lived in. Room 252 is supposedly still haunted by his ghost.

Comm 399 Assignment 2

Comm 399- 204 Question 1: a) The flow unit for the above steps is patients. b) The resources needed for: i) pre-surgery examination and orientation are the blood and urine test equipment, dinner supplies, tea and cookies and a bed. ii) surgery are the anesthetic, operating table, supplies, operating room, a surgeon iii) recovery are a wheel chair, cookies and tea and a bed. Question 2 a) The weekly throughput rate of the hospital is 120 patients/ week b) The average bed utilization is 450/ 630 = 71% c) | 90| 90| 90| | | | | | | | | | | | | | | | | 60| | | | 60| | | | | | | | | | | | | | | | | | | | | | 30| 30| | | | | | | | | | | | | | | Mond| Tues| Wed| Thurs| Fri| Sat| Sun| Question 3 a) The average bed utilization would be 540/ 630= 86%. b) | 90| 90| 90| 90| | | | | | | | | | | | | | | | | 60| | | | | 60| 60| | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Mond| Tues| Wed| Thurs| Fri| Sat| Sun| Question 4 a) Yes more capacity does need to be added to the faci lity. The bottleneck resource is the available beds and this directly affects the throughput rate.By increasing the number of available beds the hospital can admit more patients and operate on more of them leading to an increased throughput rate. b) My suggestion would be to first add an additional operation room so the throughput rate of operations from doctors could match that of the operating rooms available at 48 per day. Then I would suggest adding additional beds adding up to as many as 54 [ (48-30) *3 ]. This would maximize the number or patients able to be operated on and take care of, reaching the capacity rate for the different stages.

My Career Objective

In my first conflict, I indispensableness to be employed as marketing assistant for a drug Maker guild. Being a un utilize graduate with a pharmaceutical course from the university, I am quite arouse to be in the marketing division because the suppose seemed to fit to my course (a bachelors Degree in pharmaceutic). The logical argument that I want to gather in includes the trade of pharmaceutical products, taking orders from the customers, and developing product line relations with both new and octogenarian customers.Target MarketMy main shoot for market is the Pharmaceutical Companies in India, which fabrication pharmaceutical products and drugs for export to South eastern Asia. Most of the drugs argon s sure-enough(a) over the counter, as these drugs are common medicines for fever, cough, and flu. there are also generic antibiotic drug medicines such as cefalexin and amoxicillin that are sold cheap. At present, China, Ind superstarnesssia, Thailand, Philippine s, and Malaysia are the emerging pharmaceutical manufacturer and exporter in the region.In order to demonstrate my desire to bend in pharmaceutical phoner in India, I checked for line of descent opportunities in some pharmaceutical companies in India. percolate appendix A or transmission line announcements samples from India. This job opportunity requires a minimal of one-year beget but I reckon that if the credential is impressive, it may non pose problem.Personal FeaturesTo prove my outlay to the position, I would like to emphasize louvre features that describe me as a individual, and as a professional. First, I posses a straight communication skills both in person and by phone which I highly-developed during my student days as macrocosm information officer in our direct organ which I believe pull up stakes benefit the marketing de give awayment. Second, I am proficient with Macintosh, PC, and spreadsheet applications I am curiously gifted with good skills that give me an benefit over other appli tooshiets. Third, I dejection in force(p)ly manage grave look of agate line operations, including inventory management and sexual conquest maintenance. I acquired knowledge in this field of force through my on-the job training at my grand fathers business firm.Fourth, I affirm a negotiable character and I cab utilityously get a abundant with others. This enables me to have the dexterity to organize and motivate others. I believe this is weighty in the live on infinite because work in harmony with others builds teamwork, which is authorized in creating a productive work environment. Above all these, is that I posses a strong work ethic and frenzy to learn. I believe that with all this features I tin help the conjunction to spend a penny headway its changes performance as healthy as in advancing the confederations competitive advantage.What makes me different from others?I understand that there are to a greater extent appl icants to the position I desired, but I would like to point out my advantage with others, which make the difference. Aside from the fivesome features that I have which I mentioned above, I am a jolly person. I have strong sense of humor and I could easily crack jokes that can collar the guts of my peers. I am voluntary to do some technical pettifoggery shooting if necessary, correct to the extent of working on twenty-four hours to ensure effective communication facility or to seemly the necessary requirements.I am awake that this is not the kind of job I am applying but if I essential to do so, I provide be volitioning. My communication skills would be an advantage too. I have communications with a circularize of important privateity that could provide an important business link. If I will be given the chance to be part of the marketing team, I could work with these important people to advance the companies concern and goals. net income ExpectationWith regard to the pa y, I will not be so token about how much as foresighted as it is respectable. I mean not really high but not very low also. But this depends on the corporation situation. If the caller-up is multi-national I would deport salary that will commensurate to my worth in the company. I believe this is honorableifiable because multi-national company earns enormous profits and it is not a sin to demand a fair share. I will not demand higher(prenominal) salary, but just the appropriate compensation based on the prevailing salary standard.However if necessary, I am willing to accept unhorse salary if that meant developing good experience for a better job 2 to three years from now. I do not think demanding a higher salary at this point is insupportable in the sense that I have not proven yet my worth. I need to prove all that I have stated in my resume. The salary that I expect is the current minimum wage for professional.Promotional strategy ( wherefore I want This put-on)I have well-read about the job opportunity in India from the internet at Yahoo job search India. The specific location of the company is in Mumbai. I want this job because I have event interest in pharmaceutical related work. at any rate this is my field of expertise and it would be more comfortable for me if will be in this fieldwork. Perhaps what attracts me to this kind of work is that since childhood, I was fascinated by the pharmacist in their beautiful uniform that I used to see at a close drugstore in our neighborhood. I lease to work in India because I want to experience a new culture. India is one of the fastest growing economies in Asia and considered to one of the emerging hegemonic economy after the united States.What do I see my ego doing in five yearsI would say that I will hush be in this company five years from now but mayhap as the marketing head of the companys marketing department. I am quite sure that both my technical and communication skills will be even more d eveloped during the course of my employment and I expect that I can acquire new skills, particularly in management, that could provide me the opportunity for career improvement. I am confident that so long as the pinch management acknowledge my worth and justly ante up my effort, I can perform my top hat and wait true-blue to the companys goals and mission.What do I see my self in ten years?As I mentioned earlier, so long as the company recognized my worth and justly compensate my effort, I will remain loyal to the company and perform my best. In this slick I see my self-being in the top management team or mayhap a branch manager in either India or in particular Asian country. My loyalty to the company will remain until the time of my retirement. The companys trust and confidence in me as well as the fare citation of my initiative, my effort, and my skills and experience with the company will be the motivating factor behind my commitment.distributional StrategyI will make th e employer aware of the benefit that I can provide through my resume with attach application letter. I must cabal an impressive resume that presents my outstanding devising specified in terms of acquired skills, private attributes, cocurricular and community involvement, awards, and more or less importantly my work experience. By government agency of these points of competencies, I can generally ace my advantages over other applicants and in the corresponding way, the employer can easily determine why he has to select me from the many choices he has.Basically, resume and application letter is the best option for all application in making that first point of converge with the employer. This is the best way to make personal impression because this reflects your sincerity and personality.Appendix AJOB ANNOUNCEMENT FROM THE INTERNETmerchandising ExecutiveCompany Profile The company is into manufacturing of High Quality Pharmaceutical, Chemicals, Cosmetics, Beverages & Food Indu stries Machineries & Equipments. chore involves The job will include sale of pharmaceutical Machinery, follow up with client, victorious orders from clients, overall business development of the old and New clients as well. Experience 1 3 yrs Job Category merchandise / SalesMedical Marketing AssociatesCompany Profile A Pharmaceutical separate of CompanyJob involves Post Graduates/ Graduates in Medicine/ Pharmacy/ science. Experience 1 2 yrs Job Category Pharmaceutical/ Biotech Keywords Medical Marketing AssociatesMore cultivation Salary Upto Rs 1.75 lacs p.a Location Ahmedabad, Bangalore, Jaipur Date affix Source http//in.jobs.yahoo.com/jobs.php?jc=PharmaceuticalWork CitedHenry, Merrit. Constructing an astounding Resume. The Jamaica Observers. April 22, 2007. http//www.jamaicaobserver.com/news/html/20070421T210000-0500_122057_OBS_CONSTRUCTING_AN_IMPRESSIVE_R_SUM__.asp.Yahoo Job Search in India. http//in.jobs.yahoo.com/jobs.php?jc=Pharmaceutical